rat anti spi 1 50 Search Results


aifm3 antibody - bsa free  (Bio-Techne corporation)
93
Bio-Techne corporation aifm3 antibody - bsa free
Aifm3 Antibody Bsa Free, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
aifm3 antibody - bsa free - by Bioz Stars, 2026-09
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mouse s100a8 antibody  (Bio-Techne corporation)
99
Bio-Techne corporation mouse s100a8 antibody
Mouse S100a8 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+anti+spi+1+50/Mouse+S100A8+Antibody/custom%40af3059%4037318981
Average 99 stars, based on 1 article reviews
mouse s100a8 antibody - by Bioz Stars, 2026-09
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mca4978  (Bio-Rad)
90
Bio-Rad mca4978
Mca4978, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+anti+spi+1+50/Mouse+anti+Human+SPI1/10__7554_slash_elife__13024-434-18-28
Average 90 stars, based on 1 article reviews
mca4978 - by Bioz Stars, 2026-09
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pten  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc pten
Pten, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+anti+spi+1+50/PTEN+Rabbit+mAb/pmc03466182-47-44-46
Average 96 stars, based on 1 article reviews
pten - by Bioz Stars, 2026-09
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rat anti ha  (Proteintech)
93
Proteintech rat anti ha
Rat Anti Ha, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+anti+spi+1+50/Goat+anti-rat+IgG+(H%2BL)%2C+AP+conjugate/pmc10270687-212-120-122
Average 93 stars, based on 1 article reviews
rat anti ha - by Bioz Stars, 2026-09
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mouse s100a9 antibody  (Bio-Techne corporation)
99
Bio-Techne corporation mouse s100a9 antibody
Mouse S100a9 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
mouse s100a9 antibody - by Bioz Stars, 2026-09
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anti spi1  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc anti spi1
Anti Spi1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+anti+spi+1+50/PU%2E1+Rabbit+mAb/pmc13051965-542-42-46
Average 95 stars, based on 1 article reviews
anti spi1 - by Bioz Stars, 2026-09
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rat anti-pecam1/cd31  (Becton Dickinson)
90
Becton Dickinson rat anti-pecam1/cd31
(A) Diagram of the tissue layers between brain and skin, corresponding to the red rectangle in the coronal section through the brain and skull (upper right). (B) Cross-section of dissected dura stained for <t>PECAM1</t> (endothelial cells) and CD206 (macrophages). (C) Coronal section through cortex (lower) and overlying pia and arachnoid (upper) stained for PECAM1 and CD206. (D) Cross-scetion of the isolated arachnoid (upper panel) and the denuded brain (lower) stained for PECAM1 and CD206. (E) Dissected dura and arachnoid stained for PECAM1 and ECAD (arachnoid epithelium); arachnoid is also stained for GLUT1 (a BBB marker; bottom panel). (F) UMAP plot of combined control and infected meninges snRNAseq datasets with cell clusters differentially colored and labeled. (G) UMAP plots of separated control and infected meninges snRNAseq datasets. (H) UMAP plots, as in panel (F) showing transcripts that are highly enriched in each of 12 cell clusters (labels in each panel). (I) Dot plot showing some of the transcript abundances that most clearly discriminate among major meningeal cell types, as well as contaminating neurons and glia. Scale bars: B-E, 100 um. All tissue and data in this and other figures are from P6 mice. The immunostaining and histochemical probes in this and other figures are indicated adjacent to the corresponding panel(s), with lettering color-coded to match the corresponding fluorescent color.
Rat Anti Pecam1/Cd31, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+anti+spi+1+50/anti+cd56/bio_rxiv__2023__01__11__523597-155-51-53
Average 90 stars, based on 1 article reviews
rat anti-pecam1/cd31 - by Bioz Stars, 2026-09
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rat anti cd206 mrc1  (Bio-Rad)
96
Bio-Rad rat anti cd206 mrc1
(A) Diagram of the tissue layers between brain and skin, corresponding to the red rectangle in the coronal section through the brain and skull (upper right). (B) Cross-section of dissected dura stained for <t>PECAM1</t> (endothelial cells) and CD206 (macrophages). (C) Coronal section through cortex (lower) and overlying pia and arachnoid (upper) stained for PECAM1 and CD206. (D) Cross-scetion of the isolated arachnoid (upper panel) and the denuded brain (lower) stained for PECAM1 and CD206. (E) Dissected dura and arachnoid stained for PECAM1 and ECAD (arachnoid epithelium); arachnoid is also stained for GLUT1 (a BBB marker; bottom panel). (F) UMAP plot of combined control and infected meninges snRNAseq datasets with cell clusters differentially colored and labeled. (G) UMAP plots of separated control and infected meninges snRNAseq datasets. (H) UMAP plots, as in panel (F) showing transcripts that are highly enriched in each of 12 cell clusters (labels in each panel). (I) Dot plot showing some of the transcript abundances that most clearly discriminate among major meningeal cell types, as well as contaminating neurons and glia. Scale bars: B-E, 100 um. All tissue and data in this and other figures are from P6 mice. The immunostaining and histochemical probes in this and other figures are indicated adjacent to the corresponding panel(s), with lettering color-coded to match the corresponding fluorescent color.
Rat Anti Cd206 Mrc1, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+anti+spi+1+50/Rat+anti+Mouse+CD206/bio_rxiv__2023__01__11__523597-155-15-17
Average 96 stars, based on 1 article reviews
rat anti cd206 mrc1 - by Bioz Stars, 2026-09
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spi1 pu 1 polyclonal antibody  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc spi1 pu 1 polyclonal antibody
(A) Diagram of the tissue layers between brain and skin, corresponding to the red rectangle in the coronal section through the brain and skull (upper right). (B) Cross-section of dissected dura stained for <t>PECAM1</t> (endothelial cells) and CD206 (macrophages). (C) Coronal section through cortex (lower) and overlying pia and arachnoid (upper) stained for PECAM1 and CD206. (D) Cross-scetion of the isolated arachnoid (upper panel) and the denuded brain (lower) stained for PECAM1 and CD206. (E) Dissected dura and arachnoid stained for PECAM1 and ECAD (arachnoid epithelium); arachnoid is also stained for GLUT1 (a BBB marker; bottom panel). (F) UMAP plot of combined control and infected meninges snRNAseq datasets with cell clusters differentially colored and labeled. (G) UMAP plots of separated control and infected meninges snRNAseq datasets. (H) UMAP plots, as in panel (F) showing transcripts that are highly enriched in each of 12 cell clusters (labels in each panel). (I) Dot plot showing some of the transcript abundances that most clearly discriminate among major meningeal cell types, as well as contaminating neurons and glia. Scale bars: B-E, 100 um. All tissue and data in this and other figures are from P6 mice. The immunostaining and histochemical probes in this and other figures are indicated adjacent to the corresponding panel(s), with lettering color-coded to match the corresponding fluorescent color.
Spi1 Pu 1 Polyclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+anti+spi+1+50/PU%2E1+Antibody/pmc03564041-221-24-29
Average 96 stars, based on 1 article reviews
spi1 pu 1 polyclonal antibody - by Bioz Stars, 2026-09
96/100 stars
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mlk3  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc mlk3
(A) Diagram of the tissue layers between brain and skin, corresponding to the red rectangle in the coronal section through the brain and skull (upper right). (B) Cross-section of dissected dura stained for <t>PECAM1</t> (endothelial cells) and CD206 (macrophages). (C) Coronal section through cortex (lower) and overlying pia and arachnoid (upper) stained for PECAM1 and CD206. (D) Cross-scetion of the isolated arachnoid (upper panel) and the denuded brain (lower) stained for PECAM1 and CD206. (E) Dissected dura and arachnoid stained for PECAM1 and ECAD (arachnoid epithelium); arachnoid is also stained for GLUT1 (a BBB marker; bottom panel). (F) UMAP plot of combined control and infected meninges snRNAseq datasets with cell clusters differentially colored and labeled. (G) UMAP plots of separated control and infected meninges snRNAseq datasets. (H) UMAP plots, as in panel (F) showing transcripts that are highly enriched in each of 12 cell clusters (labels in each panel). (I) Dot plot showing some of the transcript abundances that most clearly discriminate among major meningeal cell types, as well as contaminating neurons and glia. Scale bars: B-E, 100 um. All tissue and data in this and other figures are from P6 mice. The immunostaining and histochemical probes in this and other figures are indicated adjacent to the corresponding panel(s), with lettering color-coded to match the corresponding fluorescent color.
Mlk3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+anti+spi+1+50/MLK3+Antibody/pmc02998239-143-45-52
Average 93 stars, based on 1 article reviews
mlk3 - by Bioz Stars, 2026-09
93/100 stars
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rabbit anti- col25a1 (g- biosciences itt1021)  (G Biosciences)
90
G Biosciences rabbit anti- col25a1 (g- biosciences itt1021)
(A) Diagram of the tissue layers between brain and skin, corresponding to the red rectangle in the coronal section through the brain and skull (upper right). (B) Cross-section of dissected dura stained for <t>PECAM1</t> (endothelial cells) and CD206 (macrophages). (C) Coronal section through cortex (lower) and overlying pia and arachnoid (upper) stained for PECAM1 and CD206. (D) Cross-scetion of the isolated arachnoid (upper panel) and the denuded brain (lower) stained for PECAM1 and CD206. (E) Dissected dura and arachnoid stained for PECAM1 and ECAD (arachnoid epithelium); arachnoid is also stained for GLUT1 (a BBB marker; bottom panel). (F) UMAP plot of combined control and infected meninges snRNAseq datasets with cell clusters differentially colored and labeled. (G) UMAP plots of separated control and infected meninges snRNAseq datasets. (H) UMAP plots, as in panel (F) showing transcripts that are highly enriched in each of 12 cell clusters (labels in each panel). (I) Dot plot showing some of the transcript abundances that most clearly discriminate among major meningeal cell types, as well as contaminating neurons and glia. Scale bars: B-E, 100 um. All tissue and data in this and other figures are from P6 mice. The immunostaining and histochemical probes in this and other figures are indicated adjacent to the corresponding panel(s), with lettering color-coded to match the corresponding fluorescent color.
Rabbit Anti Col25a1 (G Biosciences Itt1021), supplied by G Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+anti+spi+1+50/rabbit+anti+col25a1/10__7554_slash_elife__86130-348-182-185
Average 90 stars, based on 1 article reviews
rabbit anti- col25a1 (g- biosciences itt1021) - by Bioz Stars, 2026-09
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Image Search Results


(A) Diagram of the tissue layers between brain and skin, corresponding to the red rectangle in the coronal section through the brain and skull (upper right). (B) Cross-section of dissected dura stained for PECAM1 (endothelial cells) and CD206 (macrophages). (C) Coronal section through cortex (lower) and overlying pia and arachnoid (upper) stained for PECAM1 and CD206. (D) Cross-scetion of the isolated arachnoid (upper panel) and the denuded brain (lower) stained for PECAM1 and CD206. (E) Dissected dura and arachnoid stained for PECAM1 and ECAD (arachnoid epithelium); arachnoid is also stained for GLUT1 (a BBB marker; bottom panel). (F) UMAP plot of combined control and infected meninges snRNAseq datasets with cell clusters differentially colored and labeled. (G) UMAP plots of separated control and infected meninges snRNAseq datasets. (H) UMAP plots, as in panel (F) showing transcripts that are highly enriched in each of 12 cell clusters (labels in each panel). (I) Dot plot showing some of the transcript abundances that most clearly discriminate among major meningeal cell types, as well as contaminating neurons and glia. Scale bars: B-E, 100 um. All tissue and data in this and other figures are from P6 mice. The immunostaining and histochemical probes in this and other figures are indicated adjacent to the corresponding panel(s), with lettering color-coded to match the corresponding fluorescent color.

Journal: bioRxiv

Article Title: Bacterial meningitis in the early postnatal mouse studied at single-cell resolution

doi: 10.1101/2023.01.11.523597

Figure Lengend Snippet: (A) Diagram of the tissue layers between brain and skin, corresponding to the red rectangle in the coronal section through the brain and skull (upper right). (B) Cross-section of dissected dura stained for PECAM1 (endothelial cells) and CD206 (macrophages). (C) Coronal section through cortex (lower) and overlying pia and arachnoid (upper) stained for PECAM1 and CD206. (D) Cross-scetion of the isolated arachnoid (upper panel) and the denuded brain (lower) stained for PECAM1 and CD206. (E) Dissected dura and arachnoid stained for PECAM1 and ECAD (arachnoid epithelium); arachnoid is also stained for GLUT1 (a BBB marker; bottom panel). (F) UMAP plot of combined control and infected meninges snRNAseq datasets with cell clusters differentially colored and labeled. (G) UMAP plots of separated control and infected meninges snRNAseq datasets. (H) UMAP plots, as in panel (F) showing transcripts that are highly enriched in each of 12 cell clusters (labels in each panel). (I) Dot plot showing some of the transcript abundances that most clearly discriminate among major meningeal cell types, as well as contaminating neurons and glia. Scale bars: B-E, 100 um. All tissue and data in this and other figures are from P6 mice. The immunostaining and histochemical probes in this and other figures are indicated adjacent to the corresponding panel(s), with lettering color-coded to match the corresponding fluorescent color.

Article Snippet: The following antibodies were used for tissue immunohistochemistry and immunoblotting: goat anti-CD45 (R&D Systems AF114-SP); rat anti-CD206/MRC1 (Bio-Rad MCA2235T); goat anti-CD206 (R&D Systems AP2535); rat anti-LYVE1 (Thermo Fisher/eBioscience 14-0443-82); rat anti-PU.1/Spi-1 (Novus Biologicals MAB7124); rat anti-CD180/RP105 antibody, PE (eBioscience 12-1801-81); goat anti-S100A8 (R&D Systems AF3059); rabbit anti-ERG (Cell Signaling Technologies 97249); rat anti-PECAM1/CD31 (BD Biosciences 553370); mouse anti-CLDN5, Alexa Fluor 488 conjugate (Invitrogen 352588); mouse anti-CLDN5 (Invitrogen 35-2500); rabbit anti-ZO1 (Invitrogen 40-2200); rabbit anti-LEF1 rabbit (Cell Signaling Technologies 2230); rabbit anti-LEF1, Alexa Fluor 647 conjugate (Cell Signaling Technologies 14022); rabbit anti-Occludin (Invitrogen 406100); goat anti-VEGFR2/KDR (R&D Systems AF644-SP); sheep anti-FOXP2 (R&D Systems AF5647-SP); rabbit anti-SATB2 (Abcam ab92446); rat anti-IL-6 (Biolegend 504501); rabbit anti-COL14A1 (Novus Biologicals NBP2-15940); chicken anti-GFP (Abcam Ab13970), rat anti-HA (Proteintech 7c9); rabbit anti-HA (homemade); goat anti-E-cadherin (R&D Systems AF748); rabbit anti-E-cadherin (Cell Signaling Technologies 3195); rabbit anti-AIFM3 (Novus Biologicals NBP1-76889); rabbit anti-COL25A1 (G-Biosciences ITT1021); goat anti-IGSF8 (R&D systems AF3117-SP); rabbit anti-NNAT (Abcam ab27266); mouse anti-GAPDH (Cell Signaling Technologies 97166S); rabbit anti-GAPDH (Cell Signaling Technologies 5174S); Streptavidin, Alexa Fluor 488 conjugate (Invitrogen S11223); Streptavidin, Alexa Fluor 647 conjugate (Invitrogen S32357).

Techniques: Staining, Isolation, Marker, Infection, Labeling, Immunostaining

Flatmount images of control arachnoid and dura. (A) snRNAseq UMAP plots of markers used in (B)-(D). (B) Immunostaining for macrophage markers CD206 and LYVE: a comparison between a vibratome cross-section of the cortical surface (upper panels) and an arachnoid flatmount (lower panels). In the upper panels, the cortex occupies the lower ~60% of the image, and the arachnoid occupies most of the upper 40%. (C) Immunostaining of arachnoid flatmounts for macrophage markers CD206 (cytoplasmic/surface), PU.1 (nuclear), and CD45 (cytoplasmic/surface) (upper two panels) and for EC markers OCLN, PECAM1, and ZO1 (lower six panels). (D) Flatmounts of dura, with bone attached, stained for macrophage markers CD206, CD45, and LYVE1 (top panels); EC marker PECAM1 and fibroblast markers COL14A1 (extracellular) and FOXP2 (nuclear) (central panels); and EC marker PECAM1, fibroblast marker FOXP2 (nuclear), and osteoblast marker SATB2 (nuclear) (bottom panel). Scale bars: B-D, 100 um.

Journal: bioRxiv

Article Title: Bacterial meningitis in the early postnatal mouse studied at single-cell resolution

doi: 10.1101/2023.01.11.523597

Figure Lengend Snippet: Flatmount images of control arachnoid and dura. (A) snRNAseq UMAP plots of markers used in (B)-(D). (B) Immunostaining for macrophage markers CD206 and LYVE: a comparison between a vibratome cross-section of the cortical surface (upper panels) and an arachnoid flatmount (lower panels). In the upper panels, the cortex occupies the lower ~60% of the image, and the arachnoid occupies most of the upper 40%. (C) Immunostaining of arachnoid flatmounts for macrophage markers CD206 (cytoplasmic/surface), PU.1 (nuclear), and CD45 (cytoplasmic/surface) (upper two panels) and for EC markers OCLN, PECAM1, and ZO1 (lower six panels). (D) Flatmounts of dura, with bone attached, stained for macrophage markers CD206, CD45, and LYVE1 (top panels); EC marker PECAM1 and fibroblast markers COL14A1 (extracellular) and FOXP2 (nuclear) (central panels); and EC marker PECAM1, fibroblast marker FOXP2 (nuclear), and osteoblast marker SATB2 (nuclear) (bottom panel). Scale bars: B-D, 100 um.

Article Snippet: The following antibodies were used for tissue immunohistochemistry and immunoblotting: goat anti-CD45 (R&D Systems AF114-SP); rat anti-CD206/MRC1 (Bio-Rad MCA2235T); goat anti-CD206 (R&D Systems AP2535); rat anti-LYVE1 (Thermo Fisher/eBioscience 14-0443-82); rat anti-PU.1/Spi-1 (Novus Biologicals MAB7124); rat anti-CD180/RP105 antibody, PE (eBioscience 12-1801-81); goat anti-S100A8 (R&D Systems AF3059); rabbit anti-ERG (Cell Signaling Technologies 97249); rat anti-PECAM1/CD31 (BD Biosciences 553370); mouse anti-CLDN5, Alexa Fluor 488 conjugate (Invitrogen 352588); mouse anti-CLDN5 (Invitrogen 35-2500); rabbit anti-ZO1 (Invitrogen 40-2200); rabbit anti-LEF1 rabbit (Cell Signaling Technologies 2230); rabbit anti-LEF1, Alexa Fluor 647 conjugate (Cell Signaling Technologies 14022); rabbit anti-Occludin (Invitrogen 406100); goat anti-VEGFR2/KDR (R&D Systems AF644-SP); sheep anti-FOXP2 (R&D Systems AF5647-SP); rabbit anti-SATB2 (Abcam ab92446); rat anti-IL-6 (Biolegend 504501); rabbit anti-COL14A1 (Novus Biologicals NBP2-15940); chicken anti-GFP (Abcam Ab13970), rat anti-HA (Proteintech 7c9); rabbit anti-HA (homemade); goat anti-E-cadherin (R&D Systems AF748); rabbit anti-E-cadherin (Cell Signaling Technologies 3195); rabbit anti-AIFM3 (Novus Biologicals NBP1-76889); rabbit anti-COL25A1 (G-Biosciences ITT1021); goat anti-IGSF8 (R&D systems AF3117-SP); rabbit anti-NNAT (Abcam ab27266); mouse anti-GAPDH (Cell Signaling Technologies 97166S); rabbit anti-GAPDH (Cell Signaling Technologies 5174S); Streptavidin, Alexa Fluor 488 conjugate (Invitrogen S11223); Streptavidin, Alexa Fluor 647 conjugate (Invitrogen S32357).

Techniques: Immunostaining, Staining, Marker

Changes in EC morphology and EC protein abundance and localization with infection. (A) CLDN5 localization and Sulfo-NHS-biotin leakage in arachnoid vasculature following infection or LPS administration. (B) PECAM1 localization and Sulfo-NHS-biotin leakage in arachnoid vasculature following infection or LPS administration. (C) Infection or 10 mg/kg LPS treatment increases the area covered by vasculature in flatmounts of arachnoid. (D) Immunoblotting shows a modest, but not statistically significant, reduction in CLDN5 level relative to GAPDH level in the arachnoid with infection. (E) Reduced KDR (VEGFR2) immunstaining in arachnoid ECs with infection. (F) Reduced EC nuclear LEF1 immunostaining in capillaries and veins, and reduced PECAM1 and CLDN5 staining in veins in the arachnoid with infection. (G) Immunoblotting shows a modest reduction in LEF1 level and no change in ZO1 level relative to GAPDH level in the arachnoid with infection. Scale bars: A and B, 100 um; E and F, 100 um.

Journal: bioRxiv

Article Title: Bacterial meningitis in the early postnatal mouse studied at single-cell resolution

doi: 10.1101/2023.01.11.523597

Figure Lengend Snippet: Changes in EC morphology and EC protein abundance and localization with infection. (A) CLDN5 localization and Sulfo-NHS-biotin leakage in arachnoid vasculature following infection or LPS administration. (B) PECAM1 localization and Sulfo-NHS-biotin leakage in arachnoid vasculature following infection or LPS administration. (C) Infection or 10 mg/kg LPS treatment increases the area covered by vasculature in flatmounts of arachnoid. (D) Immunoblotting shows a modest, but not statistically significant, reduction in CLDN5 level relative to GAPDH level in the arachnoid with infection. (E) Reduced KDR (VEGFR2) immunstaining in arachnoid ECs with infection. (F) Reduced EC nuclear LEF1 immunostaining in capillaries and veins, and reduced PECAM1 and CLDN5 staining in veins in the arachnoid with infection. (G) Immunoblotting shows a modest reduction in LEF1 level and no change in ZO1 level relative to GAPDH level in the arachnoid with infection. Scale bars: A and B, 100 um; E and F, 100 um.

Article Snippet: The following antibodies were used for tissue immunohistochemistry and immunoblotting: goat anti-CD45 (R&D Systems AF114-SP); rat anti-CD206/MRC1 (Bio-Rad MCA2235T); goat anti-CD206 (R&D Systems AP2535); rat anti-LYVE1 (Thermo Fisher/eBioscience 14-0443-82); rat anti-PU.1/Spi-1 (Novus Biologicals MAB7124); rat anti-CD180/RP105 antibody, PE (eBioscience 12-1801-81); goat anti-S100A8 (R&D Systems AF3059); rabbit anti-ERG (Cell Signaling Technologies 97249); rat anti-PECAM1/CD31 (BD Biosciences 553370); mouse anti-CLDN5, Alexa Fluor 488 conjugate (Invitrogen 352588); mouse anti-CLDN5 (Invitrogen 35-2500); rabbit anti-ZO1 (Invitrogen 40-2200); rabbit anti-LEF1 rabbit (Cell Signaling Technologies 2230); rabbit anti-LEF1, Alexa Fluor 647 conjugate (Cell Signaling Technologies 14022); rabbit anti-Occludin (Invitrogen 406100); goat anti-VEGFR2/KDR (R&D Systems AF644-SP); sheep anti-FOXP2 (R&D Systems AF5647-SP); rabbit anti-SATB2 (Abcam ab92446); rat anti-IL-6 (Biolegend 504501); rabbit anti-COL14A1 (Novus Biologicals NBP2-15940); chicken anti-GFP (Abcam Ab13970), rat anti-HA (Proteintech 7c9); rabbit anti-HA (homemade); goat anti-E-cadherin (R&D Systems AF748); rabbit anti-E-cadherin (Cell Signaling Technologies 3195); rabbit anti-AIFM3 (Novus Biologicals NBP1-76889); rabbit anti-COL25A1 (G-Biosciences ITT1021); goat anti-IGSF8 (R&D systems AF3117-SP); rabbit anti-NNAT (Abcam ab27266); mouse anti-GAPDH (Cell Signaling Technologies 97166S); rabbit anti-GAPDH (Cell Signaling Technologies 5174S); Streptavidin, Alexa Fluor 488 conjugate (Invitrogen S11223); Streptavidin, Alexa Fluor 647 conjugate (Invitrogen S32357).

Techniques: Infection, Western Blot, Immunostaining, Staining

Aberrant EC morphology and subcellular localization of EC proteins in the infected arachnoid. Confocal images captured with a 63x lens show the infection-induced redistribution of CLDN5 and PECAM1 in capillary ECs in arachnoid flatmounts. In each image, a large vein is on the left and several capillaries are on the right. Scale bar: 20 um.

Journal: bioRxiv

Article Title: Bacterial meningitis in the early postnatal mouse studied at single-cell resolution

doi: 10.1101/2023.01.11.523597

Figure Lengend Snippet: Aberrant EC morphology and subcellular localization of EC proteins in the infected arachnoid. Confocal images captured with a 63x lens show the infection-induced redistribution of CLDN5 and PECAM1 in capillary ECs in arachnoid flatmounts. In each image, a large vein is on the left and several capillaries are on the right. Scale bar: 20 um.

Article Snippet: The following antibodies were used for tissue immunohistochemistry and immunoblotting: goat anti-CD45 (R&D Systems AF114-SP); rat anti-CD206/MRC1 (Bio-Rad MCA2235T); goat anti-CD206 (R&D Systems AP2535); rat anti-LYVE1 (Thermo Fisher/eBioscience 14-0443-82); rat anti-PU.1/Spi-1 (Novus Biologicals MAB7124); rat anti-CD180/RP105 antibody, PE (eBioscience 12-1801-81); goat anti-S100A8 (R&D Systems AF3059); rabbit anti-ERG (Cell Signaling Technologies 97249); rat anti-PECAM1/CD31 (BD Biosciences 553370); mouse anti-CLDN5, Alexa Fluor 488 conjugate (Invitrogen 352588); mouse anti-CLDN5 (Invitrogen 35-2500); rabbit anti-ZO1 (Invitrogen 40-2200); rabbit anti-LEF1 rabbit (Cell Signaling Technologies 2230); rabbit anti-LEF1, Alexa Fluor 647 conjugate (Cell Signaling Technologies 14022); rabbit anti-Occludin (Invitrogen 406100); goat anti-VEGFR2/KDR (R&D Systems AF644-SP); sheep anti-FOXP2 (R&D Systems AF5647-SP); rabbit anti-SATB2 (Abcam ab92446); rat anti-IL-6 (Biolegend 504501); rabbit anti-COL14A1 (Novus Biologicals NBP2-15940); chicken anti-GFP (Abcam Ab13970), rat anti-HA (Proteintech 7c9); rabbit anti-HA (homemade); goat anti-E-cadherin (R&D Systems AF748); rabbit anti-E-cadherin (Cell Signaling Technologies 3195); rabbit anti-AIFM3 (Novus Biologicals NBP1-76889); rabbit anti-COL25A1 (G-Biosciences ITT1021); goat anti-IGSF8 (R&D systems AF3117-SP); rabbit anti-NNAT (Abcam ab27266); mouse anti-GAPDH (Cell Signaling Technologies 97166S); rabbit anti-GAPDH (Cell Signaling Technologies 5174S); Streptavidin, Alexa Fluor 488 conjugate (Invitrogen S11223); Streptavidin, Alexa Fluor 647 conjugate (Invitrogen S32357).

Techniques: Infection